Scientist 1

Position: Scientist 1 (Protein Expression and Purification, Contract role)

Description
The departments of BioMolecular Research and Structural Biology, within the Protein Sciences
organization at Genentech are looking for a versatile, dynamic, self-motivated individual to join us as a Scientist 1. The selected individual will contribute towards recombinant
protein expression and purification efforts to support drug discovery activities. This role will
facilitate expression analysis of Client protein encoding constructs, by building, developing and
utilizing automated high throughput processes for expression, purification and characterization
for research and portfolio projects. The qualified candidate must be technically strong, self-
driven, passionate about science, results and detail oriented. In this collaborative role, the
successful candidate will have the opportunity to work with other scientists and group leaders
within and outside our department to fulfill protein production needs. This is an on-site role
based in South San Francisco, CA.

Job Responsibilities
Protein purification using a variety of chromatographic techniques (affinity, size
exclusion, ion exchange) for structural and biochemical studies
Screening and optimizing expression and purification of challenging membrane proteins
using detergent lysis
Using various expression platforms such as E.coli, insect cells (BEVS), and mammalian
expression platforms for small and medium scale protein production
Performing SDS-PAGE, western blot, protein characterization assays by different
biophysical and biochemical methods
Operating and maintaining High performance liquid chromatography systems (HPLC) for
protein purification and analysis
Performing gel analysis, capturing and interpreting data, uploading results to LIMS
databases and sharing with stakeholders
Communicating with and presenting to collaborators and stakeholders across the Protein
Sciences organization
Required qualifications
Applicants with a Master s degree in the Biological Sciences should have at least 3-4
years of scientific experience and applicants with a PhD degree (Biochemistry,
Biophysics, Cell Biology, Molecular Biology) should have at least 1-2 years of relevant
experience
Strong protein purification expertise in affinity and size exclusion chromatography
Strong aseptic cell culture techniques for handling routinely insect and mammalian cells
Ability to think critically, analyze results, plan experiments and carry out troubleshooting
as needed

Strong desire to learn new techniques, advance technologies related to protein
optimization and interest in implementing new expression systems.
Demonstrated ability to work independently in a team-oriented setting
Expertise with automation and high-throughput process development will be a plus
Excellent verbal and written communication skills
Excellent organizational skills