Scientist 1

Scientist 1

Location: South San Francisco, CA

Duration: 12 months

Description:

The departments of Biomolecular Research and Structural Biology, within the Protein Sciences

organization at Client are looking for a versatile, dynamic, self-motivated individual to join us as a Scientist 1.

The selected individual will contribute towards recombinant protein expression and purification efforts to support drug discovery activities. This role will facilitate expression analysis of Client protein encoding constructs, by building, developing and

utilizing automated high throughput processes for expression, purification and characterization

for research and portfolio projects.

The qualified candidate must be technically strong, self- driven, passionate about science, results and detail oriented.

In this collaborative role, the successful candidate will have the opportunity to work with other scientists and group leaders within and outside our department to fulfill protein production needs.

Job Responsibilities

Protein purification using a variety of chromatographic techniques (affinity, size exclusion, ion exchange) for structural and biochemical studies

Screening and optimizing expression and purification of challenging membrane proteins using detergent lysis

Using various expression platforms such as E.coli, insect cells (BEVS), and mammalian expression platforms for small and medium scale protein production

Performing SDS-PAGE, western blot, protein characterization assays by different biophysical and biochemical methods

Operating and maintaining High performance liquid chromatography systems (HPLC) for protein purification and analysis

Performing gel analysis, capturing and interpreting data, uploading results to LIMS databases and sharing with stakeholders

Communicating with and presenting to collaborators and stakeholders across the Protein Sciences organization

Required qualifications

Applicants with a Master's degree in the Biological Sciences should have at least 3-4

years of scientific experience and applicants with a PhD degree (Biochemistry,

Biophysics, Cell Biology, Molecular Biology) should have at least 1-2 years of relevant experience

Strong protein purification expertise in affinity and size exclusion chromatography

Strong aseptic cell culture techniques for handling routinely insect and mammalian cells

Ability to think critically, analyze results, plan experiments and carry out troubleshooting as needed

Strong desire to learn new techniques, advance technologies related to protein-optimization and interest in implementing new expression systems.

Demonstrated ability to work independently in a team-oriented setting

Expertise with automation and high-throughput process development will be a plus

Excellent verbal and written communication skills

Excellent organizational skills
As an equal opportunity employer, ICONMA provides an employment environment that supports and encourages the abilities of all persons without regard to race, color, religion, age, sex, national origin, disability status, genetics, protected veteran status, sexual orientation, gender identity or expression, or any other characteristic protected by federal, state, or local laws.